Journal: Genes & Diseases

Article Title: Bmp9 modulates cell proliferation and intercellular junctions in HERS during tooth root development

doi: 10.1016/j.gendis.2025.101777

Figure Lengend Snippet: Loss of Bmp9 hinders the differentiation and secretion of odontoblasts. (A, B) Hematoxylin-eosin staining of mandibular first molars of wild-type (WT) littermates and Bmp9 knockout (KO) mice on postnatal day 0 (PN0) (A) and at 2 weeks (B) ( n = 7). The dashed-line boxes are enlarged. The asterisks indicate the odontoblasts. (C) Immunohistochemical staining of nestin in the mandibular first molars of WT littermates and Bmp9 -KO mice at 2 weeks of age ( n = 6). The dashed-line boxes are enlarged. The asterisks indicate the odontoblasts. (D) Sirius red staining of mandibular first molars of WT littermates and Bmp9 -KO mice at 2 weeks of age ( n = 6). The dashed-line boxes are enlarged. (E) Immunohistochemical staining of DSPP in the mandibular first molars of WT littermates and Bmp9 -KO mice at 2 weeks of age ( n = 6). The dashed-line boxes are enlarged. The asterisks indicate the odontoblasts. (F, G) Quantitative analysis of nestin and DSPP expression. Scale bars = 100 μm.

Article Snippet: Primary antibodies were applied at 4 °C overnight, including BMP9 (1:100, Thermo Fisher, PA5-11931), DSPP (1:200, Abcam, ab216892), and Nestin (1:100, Bioss, bs-0008R).

Techniques: Staining, Knock-Out, Immunohistochemical staining, Expressing